Trafficking motifs in the SARS-coronavirus nucleocapsid protein.
Identifieur interne : 003563 ( Main/Exploration ); précédent : 003562; suivant : 003564Trafficking motifs in the SARS-coronavirus nucleocapsid protein.
Auteurs : Jae-Hwan You [Royaume-Uni] ; Mark L. Reed ; Julian A. HiscoxSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2007.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Fractions subcellulaires, Protéines de la matrice virale.
- physiologie : Transport de protéines.
- Animaux, Cellules COS, Liaison aux protéines, Motifs d'acides aminés, Sites de fixation.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Viral Matrix Proteins.
- metabolism : Subcellular Fractions.
- physiology : Protein Transport.
- Amino Acid Motifs, Animals, Binding Sites, COS Cells, Chlorocebus aethiops, Protein Binding.
Abstract
The severe acute respiratory syndrome-coronavirus nucleocapsid (N) protein is involved in virus replication and modulation of cell processes. In this latter respect control may in part be achieved through the sub-cellular localisation of the protein. N protein predominately localises in the cytoplasm (the site of virus replication and assembly) but also in the nucleus/nucleolus. Using a combination of live-cell and confocal microscopy coupled to mutagenesis we identified a cryptic nucleolar localisation signal in the central part of the N protein. In addition, based on structural comparison to the avian coronavirus N protein, a nuclear export signal was identified in the C-terminal region of the protein.
DOI: 10.1016/j.bbrc.2007.05.036
PubMed: 17524366
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001E04
- to stream PubMed, to step Curation: 001E04
- to stream PubMed, to step Checkpoint: 001C46
- to stream Ncbi, to step Merge: 001976
- to stream Ncbi, to step Curation: 001976
- to stream Ncbi, to step Checkpoint: 001976
- to stream Main, to step Merge: 003672
- to stream Main, to step Curation: 003563
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Trafficking motifs in the SARS-coronavirus nucleocapsid protein.</title><author><name sortKey="You, Jae Hwan" sort="You, Jae Hwan" uniqKey="You J" first="Jae-Hwan" last="You">Jae-Hwan You</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT</wicri:regionArea><orgName type="university">Université de Leeds</orgName><placeName><settlement type="city">Leeds</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Yorkshire-et-Humber</region></placeName></affiliation></author><author><name sortKey="Reed, Mark L" sort="Reed, Mark L" uniqKey="Reed M" first="Mark L" last="Reed">Mark L. Reed</name></author><author><name sortKey="Hiscox, Julian A" sort="Hiscox, Julian A" uniqKey="Hiscox J" first="Julian A" last="Hiscox">Julian A. Hiscox</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17524366</idno><idno type="pmid">17524366</idno><idno type="doi">10.1016/j.bbrc.2007.05.036</idno><idno type="wicri:Area/PubMed/Corpus">001E04</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001E04</idno><idno type="wicri:Area/PubMed/Curation">001E04</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001E04</idno><idno type="wicri:Area/PubMed/Checkpoint">001C46</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001C46</idno><idno type="wicri:Area/Ncbi/Merge">001976</idno><idno type="wicri:Area/Ncbi/Curation">001976</idno><idno type="wicri:Area/Ncbi/Checkpoint">001976</idno><idno type="wicri:doubleKey">0006-291X:2007:You J:trafficking:motifs:in</idno><idno type="wicri:Area/Main/Merge">003672</idno><idno type="wicri:Area/Main/Curation">003563</idno><idno type="wicri:Area/Main/Exploration">003563</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Trafficking motifs in the SARS-coronavirus nucleocapsid protein.</title><author><name sortKey="You, Jae Hwan" sort="You, Jae Hwan" uniqKey="You J" first="Jae-Hwan" last="You">Jae-Hwan You</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Institute of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT</wicri:regionArea><orgName type="university">Université de Leeds</orgName><placeName><settlement type="city">Leeds</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Yorkshire-et-Humber</region></placeName></affiliation></author><author><name sortKey="Reed, Mark L" sort="Reed, Mark L" uniqKey="Reed M" first="Mark L" last="Reed">Mark L. Reed</name></author><author><name sortKey="Hiscox, Julian A" sort="Hiscox, Julian A" uniqKey="Hiscox J" first="Julian A" last="Hiscox">Julian A. Hiscox</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs</term><term>Animals</term><term>Binding Sites</term><term>COS Cells</term><term>Chlorocebus aethiops</term><term>Protein Binding</term><term>Protein Transport (physiology)</term><term>Subcellular Fractions (metabolism)</term><term>Viral Matrix Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules COS</term><term>Fractions subcellulaires (métabolisme)</term><term>Liaison aux protéines</term><term>Motifs d'acides aminés</term><term>Protéines de la matrice virale (métabolisme)</term><term>Sites de fixation</term><term>Transport de protéines (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Matrix Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Subcellular Fractions</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fractions subcellulaires</term><term>Protéines de la matrice virale</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Transport de protéines</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Protein Transport</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Motifs</term><term>Animals</term><term>Binding Sites</term><term>COS Cells</term><term>Chlorocebus aethiops</term><term>Protein Binding</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules COS</term><term>Liaison aux protéines</term><term>Motifs d'acides aminés</term><term>Sites de fixation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome-coronavirus nucleocapsid (N) protein is involved in virus replication and modulation of cell processes. In this latter respect control may in part be achieved through the sub-cellular localisation of the protein. N protein predominately localises in the cytoplasm (the site of virus replication and assembly) but also in the nucleus/nucleolus. Using a combination of live-cell and confocal microscopy coupled to mutagenesis we identified a cryptic nucleolar localisation signal in the central part of the N protein. In addition, based on structural comparison to the avian coronavirus N protein, a nuclear export signal was identified in the C-terminal region of the protein.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Yorkshire-et-Humber</li></region><settlement><li>Leeds</li></settlement><orgName><li>Université de Leeds</li></orgName></list><tree><noCountry><name sortKey="Hiscox, Julian A" sort="Hiscox, Julian A" uniqKey="Hiscox J" first="Julian A" last="Hiscox">Julian A. Hiscox</name><name sortKey="Reed, Mark L" sort="Reed, Mark L" uniqKey="Reed M" first="Mark L" last="Reed">Mark L. Reed</name></noCountry><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="You, Jae Hwan" sort="You, Jae Hwan" uniqKey="You J" first="Jae-Hwan" last="You">Jae-Hwan You</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003563 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003563 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:17524366
|texte= Trafficking motifs in the SARS-coronavirus nucleocapsid protein.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:17524366" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |